@article{83151,
  keywords = {Animals, Rats, Neurons, Embryo, Mammalian, Cell Culture Techniques, Pseudorabies, Herpesvirus 1, Suid, PC12 Cells, Superior Cervical Ganglion, Clinical Laboratory Techniques},
  author = {Toh Hean Ch{\textquoteright}ng and Alexander Flood and Lynn William Enquist},
  title = {Culturing primary and transformed neuronal cells for studying pseudorabies virus infection.},
  abstract = { This chapter discusses the culture of primary sympathetic neurons (superior cervical ganglia) from rat embryos and PC12 cells differentiated into neurons for use in viral infection experiments. Methods are described for the use of a neurotropic herpesvirus, pseudorabies virus (PRV), to analyze the assembly, egress, and transport of viral antigens in neurons. },
  year = {2005},
  journal = {Methods Mol Biol},
  volume = {292},
  pages = {299-316},
  issn = {1064-3745},
  language = {eng},
}