@article{83046,
  keywords = {Animals, Rats, Neurons, Cell Line, Phosphoproteins, Recombinant Fusion Proteins, Kinesin, Host-Pathogen Interactions, Cells, Cultured, Lipoproteins, Herpesvirus 1, Suid, Viral Proteins, Alphaherpesvirinae, Swine, Viral Envelope Proteins, Virion, Axonal Transport, PC12 Cells},
  author = {Radomir Kratchmarov and Tal Kramer and Todd Greco and Matthew Taylor and Toh Hean Ch{\textquoteright}ng and Ileana Cristea and Lynn Enquist},
  title = {Glycoproteins gE and gI are required for efficient KIF1A-dependent anterograde axonal transport of alphaherpesvirus particles in neurons.},
  abstract = {<p>Alphaherpesviruses, including pseudorabies virus (PRV), spread directionally within the nervous systems of their mammalian hosts. Three viral membrane proteins are required for efficient anterograde-directed spread of infection in neurons, including Us9 and a heterodimer composed of the glycoproteins gE and gI. We previously demonstrated that the kinesin-3 motor KIF1A mediates anterograde-directed transport of viral particles in axons of cultured peripheral nervous system (PNS) neurons. The PRV Us9 protein copurifies with KIF1A, recruiting the motor to transport vesicles, but at least one unidentified additional viral protein is necessary for this interaction. Here we show that gE/gI are required for efficient anterograde transport of viral particles in axons by mediating the interaction between Us9 and KIF1A. In the absence of gE/gI, viral particles containing green fluorescent protein (GFP)-tagged Us9 are assembled in the cell body but are not sorted efficiently into axons. Importantly, we found that gE/gI are necessary for efficient copurification of KIF1A with Us9, especially at early times after infection. We also constructed a PRV recombinant that expresses a functional gE-GFP fusion protein and used affinity purification coupled with mass spectrometry to identify gE-interacting proteins. Several viral and host proteins were found to associate with gE-GFP. Importantly, both gI and Us9, but not KIF1A, copurified with gE-GFP. We propose that gE/gI are required for efficient KIF1A-mediated anterograde transport of viral particles because they indirectly facilitate or stabilize the interaction between Us9 and KIF1A.</p>
},
  year = {2013},
  journal = {J Virol},
  volume = {87},
  pages = {9431-40},
  month = {09/2013},
  issn = {1098-5514},
  doi = {10.1128/JVI.01317-13},
  language = {eng},
}