@article{82971,
  keywords = {Animals, Scattering, Small Angle, Nuclear Proteins, Capsid, Nuclear Envelope, Herpesvirus 1, Human, Herpesvirus 1, Suid, Active Transport, Cell Nucleus, Cercopithecus aethiops, Cryoelectron Microscopy, Electron Microscope Tomography, Pyrimidine Dimers, Transport Vesicles, Vero Cells, Viral Proteins},
  author = {Christoph Hagen and Kyle Dent and Tzviya Zeev-Ben-Mordehai and Michael Grange and Jens Bosse and Cathy Whittle and Barbara Klupp and Alistair Siebert and Daven Vasishtan and Felix B{\"a}uerlein and Juliana Cheleski and Stephan Werner and Peter Guttmann and Stefan Rehbein and Katja Henzler and Justin Demmerle and Barbara Adler and Ulrich Koszinowski and Lothar Schermelleh and Gerd Schneider and Lynn Enquist and J{\"u}rgen Plitzko and Thomas Mettenleiter and Kay Gr{\"u}newald},
  title = {Structural Basis of Vesicle Formation at the Inner Nuclear Membrane.},
  abstract = {<p>Vesicular nucleo-cytoplasmic transport is becoming recognized as a general cellular mechanism for translocation of large cargoes across the nuclear envelope. Cargo is recruited, enveloped at the inner nuclear membrane (INM), and delivered by membrane fusion at the outer nuclear membrane. To understand the structural underpinning for this trafficking, we investigated nuclear egress of progeny herpesvirus capsids where capsid envelopment is mediated by two viral proteins, forming the nuclear egress complex (NEC). Using a multi-modal imaging approach, we visualized the NEC in\&nbsp;situ forming coated vesicles of defined size. Cellular electron cryo-tomography revealed a protein layer showing two distinct hexagonal lattices at its membrane-proximal and membrane-distant faces, respectively. NEC coat architecture was determined by combining this information with integrative modeling using small-angle X-ray scattering data. The molecular arrangement of the NEC establishes the basic mechanism for budding and scission of tailored vesicles at the INM.</p>
},
  year = {2015},
  journal = {Cell},
  volume = {163},
  pages = {1692-701},
  month = {12/2015},
  issn = {1097-4172},
  doi = {10.1016/j.cell.2015.11.029},
  language = {eng},
}